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ELISA Kit for Agouti Related Protein (AGRP) - Rat

ELISA Kit for Agouti Related Protein (AGRP) - Rat

  • Rs 46,93000


Description: 96 well plate
Catalog No: RS01E2809

Organism species : Rattus norvegicus (Rat)

Sample TYPESerum, plasma and other biological fluids.

Format : 96-well strip plate

Assay length : 4.5 hours

Detection RANGE

  • 9.38-600pg/mL The standard curve concentrations used for the ELISA’s were 600pg/mL, 300pg/mL, 150pg/mL, 75pg/mL, 37.5pg/mL, 18.75pg/mL, 9.38pg/mL

Sensitivity : 

  • The minimum detectable dose of this kit is typically less than 3.5pg/mL..
Specificity :
  • This assay has high sensitivity and excellent specificity for detection of Agouti Related Protein (AGRP). 
  • No significant cross-reactivity or interference between Agouti Related Protein (AGRP) and analogues was observed.
Precision :
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Agouti Related Protein (AGRP) were tested 20 times on one plate, respectively. 
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Agouti Related Protein (AGRP) were tested on 3 different plates, 8 replicates in each plate. 
  • CV(%) = SD/meanX100 
  • Intra-Assay: CV<10% 
  • Inter-Assay: CV<12%
                                                                Stability :
                                                                • The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. 
                                                                • To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
                                                                Reagents and materials PROVIDED :
                                                                Reagents Quantity Reagents Quantity
                                                                Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
                                                                Standard 2 Standard Diluent 1×20mL
                                                                Detection Reagent A 1×120µL Assay Diluent A 1×12mL
                                                                Detection Reagent B 1×120µL Assay Diluent B 1×12mL
                                                                TMB Substrate 1×9mL Stop Solution 1×6mL
                                                                Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1