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ELISA Kit for Afamin (AFM) - Rat

ELISA Kit for Afamin (AFM) - Rat

  • Rs 49,40000


Description: 96 well plate
Catalog No: RS01E0438

Organism species : Rattus norvegicus (Rat)

Sample type : Serum, plasma and other biological fluids.

Format : 96-well strip plate

Assay length : 2.5 hours

Detection range : 
  • 2.47-200ng/mL The standard curve concentrations used for the ELISA’s were 200ng/mL, 66.67ng/mL, 22.22ng/mL, 7.41ng/mL, 2.47ng/mL
Sensitivity : 
  • The minimum detectable dose of this kit is typically less than   0.93ng/mL.
Specificity :
  • This assay has high sensitivity and excellent specificity for detection of Afamin (AFM).
    No significant cross-reactivity or interference between Afamin (AFM) and analogues was observed.
Precision :
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arginine (Arg) were tested 20 times on one plate, respectively. 
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arginine (Arg) were tested on 3 different plates, 8 replicates in each plate. 
  • CV(%) = SD/meanX100 
  • Intra-Assay: CV<10% 
  • Inter-Assay: CV<12%
                                          Stability :
                                          • The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. 
                                          • To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end
                                          Reagents and materials provided :
                                          Reagents Quantity Reagents Quantity
                                          Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
                                          Standard 2 Standard Diluent 1×20mL
                                          Detection Reagent A 1×120µL Assay Diluent A 1×12mL
                                          TMB Substrate 1×9mL Stop Solution 1×6mL
                                          Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1