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ELISA Kit for Adenosine Deaminase (ADA) - Human

ELISA Kit for Adenosine Deaminase (ADA) - Human

  • Rs 43,22500


Description: 96 well plate
Catalog No: RS01E2922

Organism species : Homo sapiens (Human)

Sample TYPESerum, plasma and other biological fluids.

Format : 96-well strip plate

Assay length : 4.5 hours

Detection RANGE

  • 1.56-100ng/mL The standard curve concentrations used for the ELISA’s were 100ng/mL, 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.13ng/mL, 1.56ng/mL

Sensitivity : 

  • The minimum detectable dose of this kit is typically less than 0.59ng/mL.
Specificity :
  • This assay has high sensitivity and excellent specificity for detection of Adenosine Deaminase (ADA). 
  • No significant cross-reactivity or interference between Adenosine Deaminase (ADA) and analogues was observed.
Precision :
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenosine Deaminase (ADA) were tested 20 times on one plate, respectively. 
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenosine Deaminase (ADA) were tested on 3 different plates, 8 replicates in each plate. 
  • CV(%) = SD/meanX100 
  • Intra-Assay: CV<10% 
  • Inter-Assay: CV<12%
                                                                  Stability :
                                                                  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenosine Deaminase (ADA) were tested 20 times on one plate, respectively. 
                                                                  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenosine Deaminase (ADA) were tested on 3 different plates, 8 replicates in each plate. 
                                                                  • CV(%) = SD/meanX100 
                                                                  • Intra-Assay: CV<10% 
                                                                  • Inter-Assay: CV<12%
                                                                    Reagents and materials PROVIDED :
                                                                    Reagents Quantity Reagents Quantity
                                                                    Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
                                                                    Standard 2 Standard Diluent 1×20mL
                                                                    Detection Reagent A 1×120µL Assay Diluent A 1×12mL
                                                                    Detection Reagent B 1×120µL Assay Diluent B 1×12mL
                                                                    TMB Substrate 1×9mL Stop Solution 1×6mL
                                                                    Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1