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ELISA Kit for Acetylcholine (ACH) - General

ELISA Kit for Acetylcholine (ACH) - General

  • Rs 39,78000


Description: 96 well plate
Catalog No: RS01E0306

Organism species : General

Sample type : Serum, plasma and other biological fluids.

Format : 96-well strip plate

Assay length : 2.5 hours

Detection range : 
  • 12.35-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 333.33pg/mL, 111.11pg/mL, 37.04pg/mL, 12.35pg/mL
Sensitivity : 
  • The minimum detectable dose of this kit is typically less than  4.74pg/mL.
Specificity :
  • This assay has high sensitivity and excellent specificity for detection of Acetylcholine (ACH).
    No significant cross-reactivity or interference between Acetylcholine (ACH) and analogues was observed.
Precision :
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acetylcholine (ACH) were tested 20 times on one plate, respectively. 
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acetylcholine (ACH) were tested on 3 different plates, 8 replicates in each plate. 
  • CV(%) = SD/meanX100 
  • Intra-Assay: CV<10% 
  • Inter-Assay: CV<12%
                              Stability :
                              • The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. 
                              • To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
                              Reagents and materials provided :
                              Reagents Quantity Reagents Quantity
                              Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
                              Standard 2 Standard Diluent 1×20mL
                              Detection Reagent A 1 Assay Diluent A 1×12mL
                              Detection Reagent B 1×120µL Assay Diluent B 1×12mL
                              Reagent Diluent 1×300µL Stop Solution 1×6mL
                              TMB Substrate 1×9mL Instruction manual 1
                              Wash Buffer (30 × concentrate) 1×20mL